Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18331 | 3' | -53.1 | NC_004681.1 | + | 10333 | 0.74 | 0.465934 |
Target: 5'- --cCCGGAACUcuuggUCUCCAAGcAGGUugGc -3' miRNA: 3'- cguGGCCUUGA-----AGAGGUUC-UCCGugC- -5' |
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18331 | 3' | -53.1 | NC_004681.1 | + | 65481 | 0.74 | 0.445343 |
Target: 5'- gGCACCGGAucgggauACUUCUCgAGGAGG-GCu -3' miRNA: 3'- -CGUGGCCU-------UGAAGAGgUUCUCCgUGc -5' |
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18331 | 3' | -53.1 | NC_004681.1 | + | 39331 | 0.79 | 0.265146 |
Target: 5'- cGCGCUGGAcACggCUCCGAGGGGC-CGc -3' miRNA: 3'- -CGUGGCCU-UGaaGAGGUUCUCCGuGC- -5' |
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18331 | 3' | -53.1 | NC_004681.1 | + | 61056 | 1.1 | 0.002123 |
Target: 5'- uGCACCGGAACUUCUCCAAGAGGCACGc -3' miRNA: 3'- -CGUGGCCUUGAAGAGGUUCUCCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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