Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18332 | 3' | -55 | NC_004681.1 | + | 25243 | 0.73 | 0.407816 |
Target: 5'- gCCAUGGCGCCcGCggcggcGGUUGGGuGCGCu -3' miRNA: 3'- -GGUGUCGCGGuUGa-----CCAACUC-CGUGc -5' |
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18332 | 3' | -55 | NC_004681.1 | + | 13089 | 0.77 | 0.234343 |
Target: 5'- uCCGCGGUGCCAGC-GGUguagccggUGAGcGCACGu -3' miRNA: 3'- -GGUGUCGCGGUUGaCCA--------ACUC-CGUGC- -5' |
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18332 | 3' | -55 | NC_004681.1 | + | 61994 | 1.1 | 0.001406 |
Target: 5'- gCCACAGCGCCAACUGGUUGAGGCACGc -3' miRNA: 3'- -GGUGUCGCGGUUGACCAACUCCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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