Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18333 | 3' | -56.7 | NC_004681.1 | + | 39425 | 0.67 | 0.686385 |
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Target: 5'- -uCGGAGCCGUGuccaGCGCGGcGCCgcgCGc -3' miRNA: 3'- guGCUUCGGCAU----UGCGUCcCGGa--GCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 25060 | 0.66 | 0.695874 |
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Target: 5'- -cCGucGCCGggggugaacuggaUGAUGCGGGGCCgUCGa -3' miRNA: 3'- guGCuuCGGC-------------AUUGCGUCCCGG-AGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 6678 | 0.66 | 0.707408 |
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Target: 5'- cCAUGgcGCCcuugacGgcGCGCAGGuGCUUCGg -3' miRNA: 3'- -GUGCuuCGG------CauUGCGUCC-CGGAGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 12993 | 0.66 | 0.707408 |
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Target: 5'- gCACGAAGCCGgugugGGuguggucaccCGCAGcGGCCguaGUg -3' miRNA: 3'- -GUGCUUCGGCa----UU----------GCGUC-CCGGag-CA- -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 62339 | 0.66 | 0.707408 |
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Target: 5'- gACGAGGCCcu-GCGUuacGGCUUCGUg -3' miRNA: 3'- gUGCUUCGGcauUGCGuc-CCGGAGCA- -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 4169 | 0.66 | 0.728153 |
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Target: 5'- gCGCGggGuuG--GCGUGGGGCCagcacuccUCGUu -3' miRNA: 3'- -GUGCuuCggCauUGCGUCCCGG--------AGCA- -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 75655 | 0.66 | 0.728153 |
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Target: 5'- uGCGuuguGGCaCGUGGCGCaaAGGGCUcuaccgugUCGUg -3' miRNA: 3'- gUGCu---UCG-GCAUUGCG--UCCCGG--------AGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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