Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18333 | 3' | -56.7 | NC_004681.1 | + | 58749 | 0.73 | 0.333819 |
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Target: 5'- cCGCGGcgcaggcGGCCGUGGCGCAGGacaCCUUGa -3' miRNA: 3'- -GUGCU-------UCGGCAUUGCGUCCc--GGAGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 45082 | 0.71 | 0.42001 |
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Target: 5'- cCACGAGGCCGaggauGCaGCAGGGCUcaUUGg -3' miRNA: 3'- -GUGCUUCGGCau---UG-CGUCCCGG--AGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 45182 | 0.71 | 0.448086 |
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Target: 5'- aGCGAGaCCGcGGCG-AGGGCCUCGa -3' miRNA: 3'- gUGCUUcGGCaUUGCgUCCCGGAGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 7576 | 0.7 | 0.497071 |
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Target: 5'- -cCGGAGCgucCG-AACGCAGGGCCUgGc -3' miRNA: 3'- guGCUUCG---GCaUUGCGUCCCGGAgCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 7695 | 0.69 | 0.517335 |
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Target: 5'- gGCGAAGUCGcgGGCGUAGGuGCCaUCa- -3' miRNA: 3'- gUGCUUCGGCa-UUGCGUCC-CGG-AGca -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 69008 | 0.69 | 0.527591 |
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Target: 5'- gGCGGcGGCCGUGACGaugguCAGcGCCUCGa -3' miRNA: 3'- gUGCU-UCGGCAUUGC-----GUCcCGGAGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 75655 | 0.66 | 0.728153 |
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Target: 5'- uGCGuuguGGCaCGUGGCGCaaAGGGCUcuaccgugUCGUg -3' miRNA: 3'- gUGCu---UCG-GCAUUGCG--UCCCGG--------AGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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