Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18333 | 3' | -56.7 | NC_004681.1 | + | 45182 | 0.71 | 0.448086 |
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Target: 5'- aGCGAGaCCGcGGCG-AGGGCCUCGa -3' miRNA: 3'- gUGCUUcGGCaUUGCgUCCCGGAGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 62339 | 0.66 | 0.707408 |
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Target: 5'- gACGAGGCCcu-GCGUuacGGCUUCGUg -3' miRNA: 3'- gUGCUUCGGcauUGCGuc-CCGGAGCA- -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 31904 | 0.67 | 0.665166 |
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Target: 5'- uCGCGGcGGCCGccauCGC-GGGCCUCa- -3' miRNA: 3'- -GUGCU-UCGGCauu-GCGuCCCGGAGca -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 17801 | 0.67 | 0.654507 |
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Target: 5'- cCACGcAGGCCGacaucAACGCGGcGGCCgCGc -3' miRNA: 3'- -GUGC-UUCGGCa----UUGCGUC-CCGGaGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 36326 | 0.68 | 0.579864 |
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Target: 5'- -cCGucGAGUCGUAGUGguGGGCUUCGUg -3' miRNA: 3'- guGC--UUCGGCAUUGCguCCCGGAGCA- -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 27894 | 0.71 | 0.401002 |
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Target: 5'- gGCGGcaccaacucguccAGUagCGgcACGCGGGGCCUCGg -3' miRNA: 3'- gUGCU-------------UCG--GCauUGCGUCCCGGAGCa -5' |
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| 18333 | 3' | -56.7 | NC_004681.1 | + | 67328 | 0.79 | 0.144571 |
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Target: 5'- -uCGAccuGGCCGUGuCGCAGGGCCUgGUg -3' miRNA: 3'- guGCU---UCGGCAUuGCGUCCCGGAgCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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