Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18334 | 3' | -51.3 | NC_004681.1 | + | 48046 | 0.66 | 0.93733 |
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Target: 5'- gGggGCUCUGGaugccuucucucauuGGAgugUGAUGGACGaGGAAg -3' miRNA: 3'- -CuuCGAGGUU---------------CCU---ACUACCUGC-CUUU- -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 35359 | 0.66 | 0.929595 |
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Target: 5'- cGAGGC-CCGcuGGGUGucgGGAUGGAGc -3' miRNA: 3'- -CUUCGaGGUu-CCUACua-CCUGCCUUu -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 7496 | 0.66 | 0.929595 |
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Target: 5'- aAGGCUUCGAcGGAcu-UGGGCGGggGc -3' miRNA: 3'- cUUCGAGGUU-CCUacuACCUGCCuuU- -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 58974 | 0.67 | 0.904365 |
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Target: 5'- aGAAGCUggCGAGGA-GGUGGAUGGu-- -3' miRNA: 3'- -CUUCGAg-GUUCCUaCUACCUGCCuuu -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 30966 | 0.69 | 0.783034 |
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Target: 5'- cGggGCUCCAGcGGGUcGgcGGGCGGc-- -3' miRNA: 3'- -CuuCGAGGUU-CCUA-CuaCCUGCCuuu -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 48303 | 0.73 | 0.56289 |
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Target: 5'- aGAAGCUCUggGAGGA-GAUGGACGaGGc- -3' miRNA: 3'- -CUUCGAGG--UUCCUaCUACCUGC-CUuu -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 30147 | 0.76 | 0.436063 |
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Target: 5'- gGGAGCUCCGAGGccGAUGGccGCGGc-- -3' miRNA: 3'- -CUUCGAGGUUCCuaCUACC--UGCCuuu -5' |
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| 18334 | 3' | -51.3 | NC_004681.1 | + | 63089 | 1.07 | 0.004232 |
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Target: 5'- aGAAGCUCCAAGGAUGAUGGACGGAAAg -3' miRNA: 3'- -CUUCGAGGUUCCUACUACCUGCCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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