Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18335 | 3' | -61.7 | NC_004681.1 | + | 64910 | 0.66 | 0.484158 |
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Target: 5'- gGGGUGcccUCGgaGGCGGGaugcagcucguGGGGCGAGa -3' miRNA: 3'- -CCCAC---AGC--UCGCCCccuu-------CCCCGCUC- -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 30975 | 0.68 | 0.382615 |
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Target: 5'- aGGGUGUCGcggggcuccAGCGGGucGGcGGGcGGCGuGc -3' miRNA: 3'- -CCCACAGC---------UCGCCC--CCuUCC-CCGCuC- -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 6975 | 0.7 | 0.305999 |
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Target: 5'- uGGUcgacGUCGAGCGGgacucggucGGGAAGGGGUu-- -3' miRNA: 3'- cCCA----CAGCUCGCC---------CCCUUCCCCGcuc -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 49659 | 0.7 | 0.292162 |
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Target: 5'- uGGUGccUCGGgccugcccGCGGGGGAAGgccaGGGCGAu -3' miRNA: 3'- cCCAC--AGCU--------CGCCCCCUUC----CCCGCUc -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 43914 | 0.71 | 0.259719 |
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Target: 5'- uGGUGcCGuAGCGGGGGu-GGGcGCGGa -3' miRNA: 3'- cCCACaGC-UCGCCCCCuuCCC-CGCUc -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 11289 | 0.72 | 0.203701 |
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Target: 5'- aGGcUGcCGGGUGGGGGAcaGGGUGAGg -3' miRNA: 3'- cCC-ACaGCUCGCCCCCUucCCCGCUC- -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 2983 | 0.74 | 0.150583 |
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Target: 5'- aGGG-GUCGAGCGGGaGGggGGcuucgucacGGuCGAGc -3' miRNA: 3'- -CCCaCAGCUCGCCC-CCuuCC---------CC-GCUC- -5' |
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| 18335 | 3' | -61.7 | NC_004681.1 | + | 63263 | 1.09 | 0.000404 |
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Target: 5'- aGGGUGUCGAGCGGGGGAAGGGGCGAGu -3' miRNA: 3'- -CCCACAGCUCGCCCCCUUCCCCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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