Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18338 | 5' | -55.4 | NC_004681.1 | + | 50303 | 0.66 | 0.741715 |
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Target: 5'- cUGGCGaccaccuuggGGUGCUUGUGGaaguUGCGG-CUAa -3' miRNA: 3'- -ACCGCa---------CCGUGAACAUC----ACGCCuGAU- -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 22695 | 0.66 | 0.731259 |
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Target: 5'- aGGaGUGGCGCccccUGGUGUGGGCa- -3' miRNA: 3'- aCCgCACCGUGaac-AUCACGCCUGau -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 19348 | 0.67 | 0.710066 |
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Target: 5'- aGGUGaagaccUGGCGCUUGc--UGCGGACg- -3' miRNA: 3'- aCCGC------ACCGUGAACaucACGCCUGau -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 57707 | 0.67 | 0.677735 |
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Target: 5'- cGGCGgaGGgAC-UGUAGgcgGCGGACg- -3' miRNA: 3'- aCCGCa-CCgUGaACAUCa--CGCCUGau -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 3136 | 0.68 | 0.645019 |
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Target: 5'- aGGCccuUGGCGCcgcgGGUGCGGGCa- -3' miRNA: 3'- aCCGc--ACCGUGaacaUCACGCCUGau -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 53428 | 0.68 | 0.612196 |
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Target: 5'- gUGaGCGcaGGUACUUGUAGUGCuccaGGACc- -3' miRNA: 3'- -AC-CGCa-CCGUGAACAUCACG----CCUGau -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 56180 | 0.69 | 0.590383 |
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Target: 5'- cGGCGUcgcggaagaGGUugUUGUAGUGCgcguaguccacGGACa- -3' miRNA: 3'- aCCGCA---------CCGugAACAUCACG-----------CCUGau -5' |
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| 18338 | 5' | -55.4 | NC_004681.1 | + | 64283 | 1.07 | 0.001725 |
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Target: 5'- uUGGCGUGGCACUUGUAGUGCGGACUAc -3' miRNA: 3'- -ACCGCACCGUGAACAUCACGCCUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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