Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18339 | 3' | -49.9 | NC_004681.1 | + | 64765 | 0.66 | 0.968022 |
Target: 5'- -aAUCGcCUUCGggcGGUGGGCAUUCcGGu -3' miRNA: 3'- agUAGUaGAAGU---CCAUCCGUAGGaCC- -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 25540 | 0.66 | 0.964546 |
Target: 5'- cUCGuUCAgCUUCAGGUuGGUGUCCg-- -3' miRNA: 3'- -AGU-AGUaGAAGUCCAuCCGUAGGacc -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 38026 | 0.67 | 0.960813 |
Target: 5'- gUCGUUAcCUagUCGGGUAGGCA-CUaGGg -3' miRNA: 3'- -AGUAGUaGA--AGUCCAUCCGUaGGaCC- -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 1038 | 0.67 | 0.948012 |
Target: 5'- -gGUCAgggUgAGGUcGGCGUCUUGGa -3' miRNA: 3'- agUAGUagaAgUCCAuCCGUAGGACC- -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 19619 | 0.68 | 0.918621 |
Target: 5'- gCGUgGUCgaugacggcgaGGGUGGGCAagCCUGGg -3' miRNA: 3'- aGUAgUAGaag--------UCCAUCCGUa-GGACC- -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 21398 | 0.72 | 0.738197 |
Target: 5'- aCGUCGUggaCUUgCAGG-AGaGCGUCCUGGg -3' miRNA: 3'- aGUAGUA---GAA-GUCCaUC-CGUAGGACC- -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 10016 | 0.75 | 0.605501 |
Target: 5'- aUCcgCAUCUUCGGGgaAGGCGUCggccagcugCUGGg -3' miRNA: 3'- -AGuaGUAGAAGUCCa-UCCGUAG---------GACC- -5' |
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18339 | 3' | -49.9 | NC_004681.1 | + | 65779 | 1.12 | 0.003434 |
Target: 5'- uUCAUCAUCUUCAGGUAGGCAUCCUGGu -3' miRNA: 3'- -AGUAGUAGAAGUCCAUCCGUAGGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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