Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18339 | 5' | -54.1 | NC_004681.1 | + | 46783 | 0.66 | 0.808019 |
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Target: 5'- gUUgCGGGaGUcCUUGGAGAUGGCCUg -3' miRNA: 3'- -AGgGUCCgCAcGAACUUCUACUGGAa -5' |
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| 18339 | 5' | -54.1 | NC_004681.1 | + | 18092 | 0.66 | 0.798405 |
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Target: 5'- aUCCgGGGUGUGCgUGAucagcgGGAgUGGCCg- -3' miRNA: 3'- -AGGgUCCGCACGaACU------UCU-ACUGGaa -5' |
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| 18339 | 5' | -54.1 | NC_004681.1 | + | 10808 | 0.66 | 0.786637 |
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Target: 5'- -aCCAGGUGUGacacaccgugGAAGAUGAgCUUg -3' miRNA: 3'- agGGUCCGCACgaa-------CUUCUACUgGAA- -5' |
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| 18339 | 5' | -54.1 | NC_004681.1 | + | 18299 | 0.67 | 0.758305 |
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Target: 5'- aCCCGGGgGUGCgccgGGAGGcgcuccagGACCg- -3' miRNA: 3'- aGGGUCCgCACGaa--CUUCUa-------CUGGaa -5' |
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| 18339 | 5' | -54.1 | NC_004681.1 | + | 44267 | 0.69 | 0.639512 |
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Target: 5'- cCCCcGGCGUGCaUGAAGAguucACCa- -3' miRNA: 3'- aGGGuCCGCACGaACUUCUac--UGGaa -5' |
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| 18339 | 5' | -54.1 | NC_004681.1 | + | 3389 | 0.7 | 0.606362 |
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Target: 5'- gUUCAGGCGgGCgcgggacuccUUGAGGGUGGCCUUc -3' miRNA: 3'- aGGGUCCGCaCG----------AACUUCUACUGGAA- -5' |
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| 18339 | 5' | -54.1 | NC_004681.1 | + | 65744 | 1.07 | 0.002283 |
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Target: 5'- gUCCCAGGCGUGCUUGAAGAUGACCUUg -3' miRNA: 3'- -AGGGUCCGCACGAACUUCUACUGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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