Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18342 | 3' | -58.5 | NC_004681.1 | + | 57858 | 0.7 | 0.376935 |
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Target: 5'- cGGUGUCGCCUga-AGCUCGGCGa--- -3' miRNA: 3'- -CUACAGCGGAagcUCGAGCCGCgcgc -5' |
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| 18342 | 3' | -58.5 | NC_004681.1 | + | 2524 | 0.71 | 0.344205 |
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Target: 5'- ---aUCGCCUggaUUGAGCUCG-CGCGCa -3' miRNA: 3'- cuacAGCGGA---AGCUCGAGCcGCGCGc -5' |
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| 18342 | 3' | -58.5 | NC_004681.1 | + | 54152 | 0.72 | 0.30624 |
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Target: 5'- uGUGcCGCgCUUCuaGGGCUCucgGGCGCGCGg -3' miRNA: 3'- cUACaGCG-GAAG--CUCGAG---CCGCGCGC- -5' |
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| 18342 | 3' | -58.5 | NC_004681.1 | + | 51335 | 0.72 | 0.30624 |
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Target: 5'- --cGUCGCCgcgGAGCUCGGCaaGCa -3' miRNA: 3'- cuaCAGCGGaagCUCGAGCCGcgCGc -5' |
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| 18342 | 3' | -58.5 | NC_004681.1 | + | 27035 | 0.75 | 0.21177 |
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Target: 5'- cGcgGUCGCCUUCGAGUccguggUCGGCGU-CGa -3' miRNA: 3'- -CuaCAGCGGAAGCUCG------AGCCGCGcGC- -5' |
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| 18342 | 3' | -58.5 | NC_004681.1 | + | 13168 | 0.76 | 0.181599 |
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Target: 5'- aGcgG-CGgCUUCGAGCUCGGCGaUGCGa -3' miRNA: 3'- -CuaCaGCgGAAGCUCGAGCCGC-GCGC- -5' |
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| 18342 | 3' | -58.5 | NC_004681.1 | + | 67054 | 1.09 | 0.000711 |
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Target: 5'- cGAUGUCGCCUUCGAGCUCGGCGCGCGg -3' miRNA: 3'- -CUACAGCGGAAGCUCGAGCCGCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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