Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18355 | 3' | -53.3 | NC_004681.1 | + | 69620 | 0.66 | 0.894875 |
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Target: 5'- gGCC-GCCGCAGCaGUGAGcacacggCGUGGc -3' miRNA: 3'- -UGGaUGGCGUUGcCACUCaga----GCAUC- -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 46924 | 0.66 | 0.894875 |
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Target: 5'- -gUUGCCGCGG-GGcUGcGUCUCGUAGc -3' miRNA: 3'- ugGAUGGCGUUgCC-ACuCAGAGCAUC- -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 16345 | 0.66 | 0.887736 |
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Target: 5'- cAUCUccGCUGCGGCGGUGGGUggCGa-- -3' miRNA: 3'- -UGGA--UGGCGUUGCCACUCAgaGCauc -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 56239 | 0.69 | 0.768261 |
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Target: 5'- cGCCUugaUGCGGCGGUGguucuccagguagccGGUCUCGUucGGg -3' miRNA: 3'- -UGGAug-GCGUUGCCAC---------------UCAGAGCA--UC- -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 47259 | 0.69 | 0.754089 |
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Target: 5'- cGCCUACauCGCAACGaUGAaggccgGUCUCGUGa -3' miRNA: 3'- -UGGAUG--GCGUUGCcACU------CAGAGCAUc -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 8275 | 0.69 | 0.754089 |
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Target: 5'- uCCUucuucuCCGCGGCGGUcuuGGUCUCGUc- -3' miRNA: 3'- uGGAu-----GGCGUUGCCAc--UCAGAGCAuc -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 43917 | 0.69 | 0.712388 |
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Target: 5'- cGCUggUGCCGUAGCGG-GGGUgggcgcggacCUCGUGGa -3' miRNA: 3'- -UGG--AUGGCGUUGCCaCUCA----------GAGCAUC- -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 49388 | 0.7 | 0.701742 |
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Target: 5'- cGCCUugCGCGccuCGGUGuAGagcucCUCGUGGa -3' miRNA: 3'- -UGGAugGCGUu--GCCAC-UCa----GAGCAUC- -5' |
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| 18355 | 3' | -53.3 | NC_004681.1 | + | 72485 | 1.08 | 0.002715 |
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Target: 5'- gACCUACCGCAACGGUGAGUCUCGUAGu -3' miRNA: 3'- -UGGAUGGCGUUGCCACUCAGAGCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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