Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18365 | 3' | -53.6 | NC_004681.1 | + | 19344 | 0.66 | 0.820566 |
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Target: 5'- uCUGGCGUagcaCCg---GGACGUUGCCGAc -3' miRNA: 3'- uGACCGUA----GGacaaCUUGCAGCGGUU- -5' |
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| 18365 | 3' | -53.6 | NC_004681.1 | + | 15947 | 0.66 | 0.811175 |
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Target: 5'- cGCUGGCGccaUCUcGUUcauugGggUGUCGCCAu -3' miRNA: 3'- -UGACCGU---AGGaCAA-----CuuGCAGCGGUu -5' |
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| 18365 | 3' | -53.6 | NC_004681.1 | + | 44986 | 0.69 | 0.686817 |
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Target: 5'- uGCU-GCAUCCUcGgccucgUGGGCGUCGCCc- -3' miRNA: 3'- -UGAcCGUAGGA-Ca-----ACUUGCAGCGGuu -5' |
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| 18365 | 3' | -53.6 | NC_004681.1 | + | 43251 | 0.69 | 0.686817 |
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Target: 5'- cACUGGCucugagCC-GUUGAACGUgGCUAc -3' miRNA: 3'- -UGACCGua----GGaCAACUUGCAgCGGUu -5' |
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| 18365 | 3' | -53.6 | NC_004681.1 | + | 29419 | 0.69 | 0.642668 |
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Target: 5'- uCUGGCGUCaCUGgcGAG-GUCGCCu- -3' miRNA: 3'- uGACCGUAG-GACaaCUUgCAGCGGuu -5' |
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| 18365 | 3' | -53.6 | NC_004681.1 | + | 18431 | 0.7 | 0.58733 |
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Target: 5'- --gGGCAUCgCUGUccagaagggcaUGGAgGUCGCCAAc -3' miRNA: 3'- ugaCCGUAG-GACA-----------ACUUgCAGCGGUU- -5' |
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| 18365 | 3' | -53.6 | NC_004681.1 | + | 5036 | 1.06 | 0.002792 |
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Target: 5'- cACUGGCAUCCUGUUGAACGUCGCCAAg -3' miRNA: 3'- -UGACCGUAGGACAACUUGCAGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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