Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18366 | 3' | -52 | NC_004681.1 | + | 38766 | 0.66 | 0.929845 |
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Target: 5'- cAGA-GCucaAGGACUCCUggcucauggaACUGCgggccGCGCGc -3' miRNA: 3'- -UCUaCG---UCCUGAGGA----------UGAUGa----UGCGC- -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 61815 | 0.66 | 0.911768 |
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Target: 5'- cAGAaGCuGGAgCUCCUGCgcaaccACUACGUc -3' miRNA: 3'- -UCUaCGuCCU-GAGGAUGa-----UGAUGCGc -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 35909 | 0.66 | 0.905188 |
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Target: 5'- uGAgGCGGGcgaaguACUCCUGCgcagGCUggccGCGCGc -3' miRNA: 3'- uCUaCGUCC------UGAGGAUGa---UGA----UGCGC- -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 64954 | 0.67 | 0.891208 |
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Target: 5'- uGGA-GCuGGACgUCUACUacccgcacgACUACGCGa -3' miRNA: 3'- -UCUaCGuCCUGaGGAUGA---------UGAUGCGC- -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 67486 | 0.69 | 0.81589 |
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Target: 5'- uGA-GCGGGACgaUUCUGuacggcCUGCUGCGCGa -3' miRNA: 3'- uCUaCGUCCUG--AGGAU------GAUGAUGCGC- -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 34564 | 0.69 | 0.786947 |
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Target: 5'- cGGUGU-GGACUUCUucgGCUugUGCGCc -3' miRNA: 3'- uCUACGuCCUGAGGA---UGAugAUGCGc -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 14178 | 0.7 | 0.714254 |
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Target: 5'- cGcgGCGGcGGCUUCUGCgcgcaGCUugGCGa -3' miRNA: 3'- uCuaCGUC-CUGAGGAUGa----UGAugCGC- -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 68554 | 0.71 | 0.703448 |
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Target: 5'- gAGggGCAGGACUaCUUGCUGgcACGCc -3' miRNA: 3'- -UCuaCGUCCUGA-GGAUGAUgaUGCGc -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 17430 | 0.75 | 0.47603 |
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Target: 5'- aAGGUGCGGGACUUCU--UGCccACGCGg -3' miRNA: 3'- -UCUACGUCCUGAGGAugAUGa-UGCGC- -5' |
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| 18366 | 3' | -52 | NC_004681.1 | + | 5221 | 1.09 | 0.003241 |
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Target: 5'- cAGAUGCAGGACUCCUACUACUACGCGc -3' miRNA: 3'- -UCUACGUCCUGAGGAUGAUGAUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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