Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18369 | 3' | -55.4 | NC_004681.1 | + | 16698 | 0.68 | 0.700087 |
Target: 5'- cCGGCCUGU-UCCCggGUauGACGggCGGUg -3' miRNA: 3'- -GCCGGGCAuAGGGaaCA--CUGUa-GCCA- -5' |
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18369 | 3' | -55.4 | NC_004681.1 | + | 63935 | 0.68 | 0.66721 |
Target: 5'- gCGGUgaacaugaccucuUCGUcGUCCUcgGUGACGUCGGUa -3' miRNA: 3'- -GCCG-------------GGCA-UAGGGaaCACUGUAGCCA- -5' |
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18369 | 3' | -55.4 | NC_004681.1 | + | 49288 | 0.7 | 0.551048 |
Target: 5'- uCGuGCCgG-AUUCCUUGUGguACAUCGGUg -3' miRNA: 3'- -GC-CGGgCaUAGGGAACAC--UGUAGCCA- -5' |
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18369 | 3' | -55.4 | NC_004681.1 | + | 7656 | 1.08 | 0.00176 |
Target: 5'- cCGGCCCGUAUCCCUUGUGACAUCGGUg -3' miRNA: 3'- -GCCGGGCAUAGGGAACACUGUAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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