Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18372 | 5' | -53.6 | NC_004681.1 | + | 64782 | 0.66 | 0.87395 |
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Target: 5'- aGUCCgUGGCCAUCACaCGAgccuuGGGUa- -3' miRNA: 3'- -CGGG-ACUGGUAGUG-GCUacu--UCCAga -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 38802 | 0.66 | 0.87395 |
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Target: 5'- aCCCUGGCgAUgGCCGGaaGGAGuGUCa -3' miRNA: 3'- cGGGACUGgUAgUGGCUa-CUUC-CAGa -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 10502 | 0.66 | 0.887447 |
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Target: 5'- uCCUUGACCcaGUCGCCGGgagucgacggagGAcGGUCa -3' miRNA: 3'- cGGGACUGG--UAGUGGCUa-----------CUuCCAGa -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 27498 | 0.66 | 0.888897 |
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Target: 5'- aCCCUGACCuggggcaCGCCucaGAUcGAGGUCg -3' miRNA: 3'- cGGGACUGGua-----GUGG---CUAcUUCCAGa -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 48442 | 0.66 | 0.888897 |
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Target: 5'- aCCUuguUGGCCAcguccauuucgaUCACCGGgguuccgccguUGAGGGUCa -3' miRNA: 3'- cGGG---ACUGGU------------AGUGGCU-----------ACUUCCAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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