miRNA: 3'- -CGGGa-CUGGUa-GUGGCUacuUCCAGa -5'
miRNA: 3'- -CGGGac---UGGUaGUGGCUACUuCCAGa -5'
miRNA: 3'- -CGGGACuGGUAGUGGCUACUU-----------CCAGa -5'
miRNA: 3'- -CGGGAcUGGUAGUGGCUACUUCCaga -5'
miRNA: 3'- -CGGGACUGGUAGUGGCUACUUCCAGA- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18372 | 5' | -53.6 | NC_004681.1 | + | 18116 | 0.7 | 0.649667 |
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Target: 5'- cGCCCccGGCCAggCACCGGgucAGGUCg -3' miRNA: 3'- -CGGGa-CUGGUa-GUGGCUacuUCCAGa -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 50998 | 0.73 | 0.48983 |
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Target: 5'- cGCCCgcgccGCCGaCGCCGAUGA-GGUCg -3' miRNA: 3'- -CGGGac---UGGUaGUGGCUACUuCCAGa -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 53778 | 0.77 | 0.296067 |
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Target: 5'- cGCCUUG-CCAUCGCCGAUGAAcacuuggacuuGGUUg -3' miRNA: 3'- -CGGGACuGGUAGUGGCUACUU-----------CCAGa -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 50959 | 0.77 | 0.288827 |
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Target: 5'- cGCCCUcGCCAUUGCUGGUGAGGGa-- -3' miRNA: 3'- -CGGGAcUGGUAGUGGCUACUUCCaga -5' |
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| 18372 | 5' | -53.6 | NC_004681.1 | + | 8804 | 1.11 | 0.001659 |
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Target: 5'- cGCCCUGACCAUCACCGAUGAAGGUCUg -3' miRNA: 3'- -CGGGACUGGUAGUGGCUACUUCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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