Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18372 | 5' | -53.6 | NC_004681.1 | + | 14784 | 0.69 | 0.703605 |
Target: 5'- cGCCCUGGCCAUC-CUGA---AGG-CUg -3' miRNA: 3'- -CGGGACUGGUAGuGGCUacuUCCaGA- -5' |
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18372 | 5' | -53.6 | NC_004681.1 | + | 10502 | 0.66 | 0.887447 |
Target: 5'- uCCUUGACCcaGUCGCCGGgagucgacggagGAcGGUCa -3' miRNA: 3'- cGGGACUGG--UAGUGGCUa-----------CUuCCAGa -5' |
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18372 | 5' | -53.6 | NC_004681.1 | + | 9520 | 0.66 | 0.858024 |
Target: 5'- -aCC-GGCCAUCACaag-GAAGGUCg -3' miRNA: 3'- cgGGaCUGGUAGUGgcuaCUUCCAGa -5' |
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18372 | 5' | -53.6 | NC_004681.1 | + | 8804 | 1.11 | 0.001659 |
Target: 5'- cGCCCUGACCAUCACCGAUGAAGGUCUg -3' miRNA: 3'- -CGGGACUGGUAGUGGCUACUUCCAGA- -5' |
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18372 | 5' | -53.6 | NC_004681.1 | + | 721 | 0.68 | 0.752772 |
Target: 5'- uGCUCUGcACCAcgguucucuucuggUCugCGGUGAGGG-CUa -3' miRNA: 3'- -CGGGAC-UGGU--------------AGugGCUACUUCCaGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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