Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18373 | 5' | -58 | NC_004681.1 | + | 6999 | 0.67 | 0.578955 |
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Target: 5'- -aCGguggUGGCCAcGGACAUgccgugGUCGaCGUCg -3' miRNA: 3'- aaGCa---GCCGGU-CCUGUGa-----CAGC-GCAG- -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 46321 | 0.68 | 0.537178 |
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Target: 5'- aUCGUgGGCCAGG-CACUGUggaaGCu-- -3' miRNA: 3'- aAGCAgCCGGUCCuGUGACAg---CGcag -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 48289 | 0.69 | 0.476672 |
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Target: 5'- cUUC-UUGGCCAGGuauuCGCUG-CGCGUUu -3' miRNA: 3'- -AAGcAGCCGGUCCu---GUGACaGCGCAG- -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 60663 | 0.69 | 0.466911 |
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Target: 5'- gUUCGUCGGCCAGcGGC-CcGagGCGUg -3' miRNA: 3'- -AAGCAGCCGGUC-CUGuGaCagCGCAg -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 6419 | 0.7 | 0.410655 |
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Target: 5'- -gCGacCGGCCAGcACucCUGUCGCGUCu -3' miRNA: 3'- aaGCa-GCCGGUCcUGu-GACAGCGCAG- -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 50573 | 0.71 | 0.34177 |
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Target: 5'- --aGUUGGCgCAGGACACgGUCgggguggGCGUCg -3' miRNA: 3'- aagCAGCCG-GUCCUGUGaCAG-------CGCAG- -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 52387 | 0.74 | 0.237881 |
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Target: 5'- gUCGUCGGCCuucuggaggaggAGGACAacGUCcGCGUCg -3' miRNA: 3'- aAGCAGCCGG------------UCCUGUgaCAG-CGCAG- -5' |
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| 18373 | 5' | -58 | NC_004681.1 | + | 9052 | 1.07 | 0.000959 |
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Target: 5'- gUUCGUCGGCCAGGACACUGUCGCGUCg -3' miRNA: 3'- -AAGCAGCCGGUCCUGUGACAGCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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