Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18376 | 3' | -57 | NC_004681.1 | + | 24181 | 0.66 | 0.725378 |
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Target: 5'- gGGGUGGggaUGACG-UCG-GAGGGcuGGUCu -3' miRNA: 3'- -UCCAUCg--ACUGCaAGCaCUCCC--CCGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 50351 | 0.66 | 0.704738 |
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Target: 5'- uGGUcgacaaccGGCUGugGgaCGUggucGAGGGcGGCUa -3' miRNA: 3'- uCCA--------UCGACugCaaGCA----CUCCC-CCGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 31643 | 0.67 | 0.662746 |
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Target: 5'- cAGGUcuccAGCgGuCGUUgGUGAuuGGGGcGCCg -3' miRNA: 3'- -UCCA----UCGaCuGCAAgCACU--CCCC-CGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 16615 | 0.67 | 0.641551 |
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Target: 5'- gAGG-AGCUGACGUUCuGgaagcugGAcgagccgcccGGGaGGCCg -3' miRNA: 3'- -UCCaUCGACUGCAAG-Ca------CU----------CCC-CCGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 32540 | 0.68 | 0.620325 |
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Target: 5'- uGGUGgccGCUGGCaucUUCGacgcccUGaAGGGGGCCg -3' miRNA: 3'- uCCAU---CGACUGc--AAGC------AC-UCCCCCGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 30364 | 0.68 | 0.606544 |
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Target: 5'- aGGGUGGCcagcucggagugGACGcugUCGUucaucaccacaccGAGGGcGGCCa -3' miRNA: 3'- -UCCAUCGa-----------CUGCa--AGCA-------------CUCCC-CCGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 12649 | 0.69 | 0.516018 |
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Target: 5'- ---gGGCUGACGg-CGUGGauGGGGGCa -3' miRNA: 3'- uccaUCGACUGCaaGCACU--CCCCCGg -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 14243 | 0.71 | 0.401495 |
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Target: 5'- uGGGUGcagucaauguGCUuccuUGUUCG-GAGGGGGCCa -3' miRNA: 3'- -UCCAU----------CGAcu--GCAAGCaCUCCCCCGG- -5' |
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| 18376 | 3' | -57 | NC_004681.1 | + | 9641 | 1.12 | 0.000654 |
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Target: 5'- uAGGUAGCUGACGUUCGUGAGGGGGCCg -3' miRNA: 3'- -UCCAUCGACUGCAAGCACUCCCCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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