Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18377 | 5' | -58.8 | NC_004681.1 | + | 22375 | 0.66 | 0.635675 |
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Target: 5'- gGGC-AGGGCGCccacgCCGcCaCGCGGa -3' miRNA: 3'- -CCGuUCCCGCGcuucaGGCuG-GCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 41962 | 0.66 | 0.635675 |
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Target: 5'- uGCGAGGuagGCGUGGgacaGGUCgGcuCCGCGGa -3' miRNA: 3'- cCGUUCC---CGCGCU----UCAGgCu-GGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 7169 | 0.66 | 0.635675 |
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Target: 5'- cGGUggGGG-GUGccGUCCuucauggcGCCGCGGu -3' miRNA: 3'- -CCGuuCCCgCGCuuCAGGc-------UGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 5924 | 0.66 | 0.642921 |
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Target: 5'- uGGUggaaGAGGGCaUGGAGcCCauuccgcugcaguuGACCGCGGu -3' miRNA: 3'- -CCG----UUCCCGcGCUUCaGG--------------CUGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 47536 | 0.66 | 0.646025 |
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Target: 5'- uGGCu-GGGUGCGcuGcCCGgAUUGCGGu -3' miRNA: 3'- -CCGuuCCCGCGCuuCaGGC-UGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 22369 | 0.66 | 0.653264 |
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Target: 5'- cGGCGAGGaccuccccGaCGUcGAGUggacgcuggucaccCCGGCCGCGGa -3' miRNA: 3'- -CCGUUCC--------C-GCGcUUCA--------------GGCUGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 17610 | 0.66 | 0.656364 |
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Target: 5'- cGGCucGGGCGCGGA---CGAccCCGCGu -3' miRNA: 3'- -CCGuuCCCGCGCUUcagGCU--GGCGCc -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 16524 | 0.66 | 0.665652 |
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Target: 5'- uGGCGGGuGGCgGCaacGGUCCccacggcGGCCGUGGu -3' miRNA: 3'- -CCGUUC-CCG-CGcu-UCAGG-------CUGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 59611 | 0.66 | 0.666682 |
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Target: 5'- uGGCGucaccAGGGCGCucGAcaUCCGGCacguccacaaGCGGg -3' miRNA: 3'- -CCGU-----UCCCGCG--CUucAGGCUGg---------CGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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