Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18377 | 5' | -58.8 | NC_004681.1 | + | 47536 | 0.66 | 0.646025 |
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Target: 5'- uGGCu-GGGUGCGcuGcCCGgAUUGCGGu -3' miRNA: 3'- -CCGuuCCCGCGCuuCaGGC-UGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 49570 | 0.67 | 0.604641 |
|
Target: 5'- gGGCAguAGGGCuuGccGUCCuuguagcgggGGCCGUGGa -3' miRNA: 3'- -CCGU--UCCCGcgCuuCAGG----------CUGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 50411 | 0.68 | 0.543324 |
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Target: 5'- -uCGGGGGUGCccauGAGUucagcgUCGGCCGCGGg -3' miRNA: 3'- ccGUUCCCGCGc---UUCA------GGCUGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 52314 | 0.67 | 0.614975 |
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Target: 5'- aGGUcGGGGaugGCGggG-CCGuucuugGCCGUGGa -3' miRNA: 3'- -CCGuUCCCg--CGCuuCaGGC------UGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 56281 | 0.69 | 0.503581 |
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Target: 5'- gGGCAugAGGaGCGCGcuGGacuUgUGGCCGCGGa -3' miRNA: 3'- -CCGU--UCC-CGCGCu-UC---AgGCUGGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 58402 | 0.67 | 0.604641 |
|
Target: 5'- cGCGuGGGCaaGAAGUCCcgcaccuucuCCGCGGc -3' miRNA: 3'- cCGUuCCCGcgCUUCAGGcu--------GGCGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 59163 | 0.67 | 0.573793 |
|
Target: 5'- aGGCAAGGGUuCGcAGGUUCG-CCaCGGu -3' miRNA: 3'- -CCGUUCCCGcGC-UUCAGGCuGGcGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 59611 | 0.66 | 0.666682 |
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Target: 5'- uGGCGucaccAGGGCGCucGAcaUCCGGCacguccacaaGCGGg -3' miRNA: 3'- -CCGU-----UCCCGCG--CUucAGGCUGg---------CGCC- -5' |
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| 18377 | 5' | -58.8 | NC_004681.1 | + | 75531 | 0.69 | 0.493839 |
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Target: 5'- aGGCAcguGGGCuGCGccgcGAGUaCGGCCGCGcGg -3' miRNA: 3'- -CCGUu--CCCG-CGC----UUCAgGCUGGCGC-C- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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