Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18378 | 3' | -47.3 | NC_004681.1 | + | 64888 | 0.71 | 0.927494 |
Target: 5'- aGCgugu-GGGUGCGGGAGUGcgggUGGGg -3' miRNA: 3'- -CGauuucUCCACGUUCUCAUa---ACCCa -5' |
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18378 | 3' | -47.3 | NC_004681.1 | + | 30471 | 0.73 | 0.844237 |
Target: 5'- cGCcuuGGAGGUGCGGGAGUAU-GGc- -3' miRNA: 3'- -CGauuUCUCCACGUUCUCAUAaCCca -5' |
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18378 | 3' | -47.3 | NC_004681.1 | + | 16331 | 0.75 | 0.76481 |
Target: 5'- ----uGGAGGUGCAgaGGGGUGggGGGUu -3' miRNA: 3'- cgauuUCUCCACGU--UCUCAUaaCCCA- -5' |
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18378 | 3' | -47.3 | NC_004681.1 | + | 10895 | 1.12 | 0.00583 |
Target: 5'- aGCUAAAGAGGUGCAAGAGUAUUGGGUg -3' miRNA: 3'- -CGAUUUCUCCACGUUCUCAUAACCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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