Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18380 | 5' | -59.2 | NC_004681.1 | + | 6770 | 0.66 | 0.555216 |
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Target: 5'- aAGGACUacggCCCCaaUGGCgGCUUCa- -3' miRNA: 3'- cUCCUGAa---GGGGccACCGgCGAAGau -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 22902 | 0.66 | 0.534529 |
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Target: 5'- -cGGACaucUUCCUGGUGGCCG-UUCa- -3' miRNA: 3'- cuCCUGa--AGGGGCCACCGGCgAAGau -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 59613 | 0.67 | 0.504037 |
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Target: 5'- uGGGAUUUCCgCCGaaggcGUGGCCG-UUCUGc -3' miRNA: 3'- cUCCUGAAGG-GGC-----CACCGGCgAAGAU- -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 29493 | 0.68 | 0.455006 |
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Target: 5'- cGGGACcaCCUCGGUGGCUGUcacgUUCg- -3' miRNA: 3'- cUCCUGaaGGGGCCACCGGCG----AAGau -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 32528 | 0.68 | 0.436125 |
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Target: 5'- uGGGugagUUCCUGGUGGCCGCUggcaUCUu -3' miRNA: 3'- cUCCuga-AGGGGCCACCGGCGA----AGAu -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 44753 | 0.69 | 0.390979 |
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Target: 5'- cGAGGACUcCCUCGcG-GGCgGCUUCa- -3' miRNA: 3'- -CUCCUGAaGGGGC-CaCCGgCGAAGau -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 16303 | 0.69 | 0.390979 |
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Target: 5'- cGAGGGCaUCCCCGc-GGCCGgaUCg- -3' miRNA: 3'- -CUCCUGaAGGGGCcaCCGGCgaAGau -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 34539 | 0.7 | 0.348994 |
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Target: 5'- -cGGGCg-CCgCGGUGGCgCGCUUCUu -3' miRNA: 3'- cuCCUGaaGGgGCCACCG-GCGAAGAu -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 12203 | 0.72 | 0.245415 |
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Target: 5'- -cGGGCUuccccgcgcgccgcaUCCCCGGUGGCCcgUUCUGg -3' miRNA: 3'- cuCCUGA---------------AGGGGCCACCGGcgAAGAU- -5' |
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| 18380 | 5' | -59.2 | NC_004681.1 | + | 11761 | 1.06 | 0.000864 |
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Target: 5'- cGAGGACUUCCCCGGUGGCCGCUUCUAc -3' miRNA: 3'- -CUCCUGAAGGGGCCACCGGCGAAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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