Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18385 | 5' | -56.7 | NC_004681.1 | + | 2741 | 0.66 | 0.64228 |
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Target: 5'- -aCCCGGGcuaGGGUGCCguccgcGGCGUGa -3' miRNA: 3'- aaGGGUCC---UCCACGGaucua-CCGUAU- -5' |
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| 18385 | 5' | -56.7 | NC_004681.1 | + | 59285 | 0.66 | 0.63018 |
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Target: 5'- -gCCCGGGAGGccGUCcgugacaUAGGUGGCGa- -3' miRNA: 3'- aaGGGUCCUCCa-CGG-------AUCUACCGUau -5' |
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| 18385 | 5' | -56.7 | NC_004681.1 | + | 64917 | 0.66 | 0.620281 |
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Target: 5'- gUCCCAcGGGGUGCCcucGGA-GGCGg- -3' miRNA: 3'- aAGGGUcCUCCACGGa--UCUaCCGUau -5' |
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| 18385 | 5' | -56.7 | NC_004681.1 | + | 15494 | 0.66 | 0.620281 |
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Target: 5'- gUCCUuGGcggacaGGGUGCCguucUGGAUGGCGUc -3' miRNA: 3'- aAGGGuCC------UCCACGG----AUCUACCGUAu -5' |
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| 18385 | 5' | -56.7 | NC_004681.1 | + | 25426 | 0.68 | 0.533408 |
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Target: 5'- gUCUCGGGAGGgGUC--GGUGGCGUGg -3' miRNA: 3'- aAGGGUCCUCCaCGGauCUACCGUAU- -5' |
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| 18385 | 5' | -56.7 | NC_004681.1 | + | 7174 | 0.68 | 0.501869 |
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Target: 5'- gUUCCCgguGGGGGGUGCCguccuucAUGGCGc- -3' miRNA: 3'- -AAGGG---UCCUCCACGGauc----UACCGUau -5' |
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| 18385 | 5' | -56.7 | NC_004681.1 | + | 12608 | 1.04 | 0.001708 |
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Target: 5'- cUUCCCAGGAGGUGCCUAGAUGGCAUAc -3' miRNA: 3'- -AAGGGUCCUCCACGGAUCUACCGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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