Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18391 | 5' | -56.2 | NC_004681.1 | + | 39930 | 0.71 | 0.469699 |
Target: 5'- gGGCgc-GGugCGCGCggUGCGGUCCa -3' miRNA: 3'- -CCGacuCUugGCGCGuaGCGUCAGGa -5' |
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18391 | 5' | -56.2 | NC_004681.1 | + | 58478 | 0.72 | 0.385263 |
Target: 5'- aGCUGGGcGCCGCGUgaaccgcGagGCAGUCCa -3' miRNA: 3'- cCGACUCuUGGCGCG-------UagCGUCAGGa -5' |
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18391 | 5' | -56.2 | NC_004681.1 | + | 33363 | 0.75 | 0.257212 |
Target: 5'- uGGCUGAGAccgGCCGCGUcgCGCGcgCCg -3' miRNA: 3'- -CCGACUCU---UGGCGCGuaGCGUcaGGa -5' |
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18391 | 5' | -56.2 | NC_004681.1 | + | 14255 | 1.1 | 0.001012 |
Target: 5'- gGGCUGAGAACCGCGCAUCGCAGUCCUa -3' miRNA: 3'- -CCGACUCUUGGCGCGUAGCGUCAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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