Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18398 | 5' | -62.3 | NC_004681.1 | + | 29807 | 0.75 | 0.098945 |
Target: 5'- cACCAGGCGCGGGaGCUcggcauccaACgCGGCgcgcuugCCCCu -3' miRNA: 3'- -UGGUCCGCGUCC-CGA---------UG-GUCGa------GGGG- -5' |
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18398 | 5' | -62.3 | NC_004681.1 | + | 29775 | 0.76 | 0.091297 |
Target: 5'- uGCCAGGUGCAGccaGCUugCauGGCUUCCCa -3' miRNA: 3'- -UGGUCCGCGUCc--CGAugG--UCGAGGGG- -5' |
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18398 | 5' | -62.3 | NC_004681.1 | + | 16277 | 1.11 | 0.000205 |
Target: 5'- gACCAGGCGCAGGGCUACCAGCUCCCCg -3' miRNA: 3'- -UGGUCCGCGUCCCGAUGGUCGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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