Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 184 | 3' | -57.6 | AC_000007.1 | + | 15186 | 0.7 | 0.23124 |
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Target: 5'- aAGCGcGCuGAGGcCGAG-GCAGCGGCAg -3' miRNA: 3'- gUCGC-CG-UUCUcGUUCuCGUCGCCGU- -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 16243 | 0.7 | 0.213043 |
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Target: 5'- gGGCGGCAGugccGGGUcGGcGGCGGUGGCGa -3' miRNA: 3'- gUCGCCGUU----CUCGuUC-UCGUCGCCGU- -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 9389 | 0.71 | 0.185454 |
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Target: 5'- uGGCGGCGguGGGGgAGGGGggacaCGGCGGCGa -3' miRNA: 3'- gUCGCCGU--UCUCgUUCUC-----GUCGCCGU- -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 15041 | 0.73 | 0.143676 |
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Target: 5'- gGGCGGgGAuGGCGcaGGCGGCGGCAa -3' miRNA: 3'- gUCGCCgUUcUCGUucUCGUCGCCGU- -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 17573 | 0.74 | 0.110668 |
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Target: 5'- gGGCuGGCGAcGGCGAcGGCGGCGGCGg -3' miRNA: 3'- gUCG-CCGUUcUCGUUcUCGUCGCCGU- -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 2179 | 0.75 | 0.097533 |
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Target: 5'- gCGGCGGCAGGAGC-AGAGCccauggaacccgagAGcCGGCc -3' miRNA: 3'- -GUCGCCGUUCUCGuUCUCG--------------UC-GCCGu -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 26745 | 0.78 | 0.057507 |
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Target: 5'- gCAGCGGCAgcGGcAGCAAcAGCAGCGGUc -3' miRNA: 3'- -GUCGCCGU--UC-UCGUUcUCGUCGCCGu -5' |
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| 184 | 3' | -57.6 | AC_000007.1 | + | 11067 | 1.08 | 0.000284 |
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Target: 5'- gCAGCGGCAAGAGCAAGAGCAGCGGCAg -3' miRNA: 3'- -GUCGCCGUUCUCGUUCUCGUCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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