Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 184 | 5' | -59.5 | AC_000007.1 | + | 4333 | 0.66 | 0.402469 |
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Target: 5'- aGGCACCacgCCCagcgCUCCUGCUaCGa -3' miRNA: 3'- -CCGUGGga-GGGgaa-GAGGAUGGcGCa -5' |
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| 184 | 5' | -59.5 | AC_000007.1 | + | 3860 | 0.67 | 0.341363 |
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Target: 5'- uGCAgCUUCCCgUUCaUCCgcCCGCGa -3' miRNA: 3'- cCGUgGGAGGGgAAG-AGGauGGCGCa -5' |
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| 184 | 5' | -59.5 | AC_000007.1 | + | 17808 | 0.68 | 0.28653 |
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Target: 5'- cGGUAUCCUgCCC--CUCCUuauuccacugaucGCCGCGg -3' miRNA: 3'- -CCGUGGGAgGGGaaGAGGA-------------UGGCGCa -5' |
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| 184 | 5' | -59.5 | AC_000007.1 | + | 34637 | 0.69 | 0.227898 |
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Target: 5'- aGCGCCCUCCaCCUUCagCCUcaaGCaGCGa -3' miRNA: 3'- cCGUGGGAGG-GGAAGa-GGA---UGgCGCa -5' |
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| 184 | 5' | -59.5 | AC_000007.1 | + | 26895 | 0.69 | 0.227898 |
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Target: 5'- aGCGCuCCUCCuCCUgCUgCUGCCGCc- -3' miRNA: 3'- cCGUG-GGAGG-GGAaGAgGAUGGCGca -5' |
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| 184 | 5' | -59.5 | AC_000007.1 | + | 19557 | 0.7 | 0.194353 |
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Target: 5'- aGCACUCUCCC----UCCUGCCGCa- -3' miRNA: 3'- cCGUGGGAGGGgaagAGGAUGGCGca -5' |
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| 184 | 5' | -59.5 | AC_000007.1 | + | 11102 | 1.08 | 0.000264 |
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Target: 5'- gGGCACCCUCCCCUUCUCCUACCGCGUc -3' miRNA: 3'- -CCGUGGGAGGGGAAGAGGAUGGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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