Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 18404 | 3' | -56.4 | NC_004681.1 | + | 625 | 0.66 | 0.757111 |
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Target: 5'- uCGUUGAGGgacucGCGGCCGCggaAGaugGCCGgGu -3' miRNA: 3'- -GCGAUUCU-----UGCCGGCGg--UCa--UGGCgC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 10762 | 0.66 | 0.726761 |
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Target: 5'- aCGC----GACGGCCGCgGGcACCGgGg -3' miRNA: 3'- -GCGauucUUGCCGGCGgUCaUGGCgC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 39581 | 0.66 | 0.726761 |
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Target: 5'- uCGCUGccacCGGCCuugaGCCAGcgcACCGCGg -3' miRNA: 3'- -GCGAUucuuGCCGG----CGGUCa--UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 18730 | 0.66 | 0.726761 |
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Target: 5'- uCGCcAAGGgcgACGGCgGCgAG-GCCGCGc -3' miRNA: 3'- -GCGaUUCU---UGCCGgCGgUCaUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 16030 | 0.66 | 0.73698 |
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Target: 5'- uGCUGgcaAGGGC-GCCGCCGcuGUcACCGCa -3' miRNA: 3'- gCGAU---UCUUGcCGGCGGU--CA-UGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 49466 | 0.66 | 0.73698 |
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Target: 5'- cCGCUAcaAGGACGGCaagcccuacUGcCCAGUcGCCGgGg -3' miRNA: 3'- -GCGAU--UCUUGCCG---------GC-GGUCA-UGGCgC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 7187 | 0.66 | 0.744075 |
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Target: 5'- uCGCgcacGAGAagguacucgagaagGCGGCCgguGCCGGUaacggcgacACCGCGc -3' miRNA: 3'- -GCGa---UUCU--------------UGCCGG---CGGUCA---------UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 7569 | 0.66 | 0.757111 |
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Target: 5'- gGUgGAGGACGGCCG--GGgagGCCGUGa -3' miRNA: 3'- gCGaUUCUUGCCGGCggUCa--UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 19662 | 0.66 | 0.767002 |
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Target: 5'- uGCgGGGAACGGgaagCGCUGGaUGCCGCu -3' miRNA: 3'- gCGaUUCUUGCCg---GCGGUC-AUGGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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