Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18404 | 3' | -56.4 | NC_004681.1 | + | 62181 | 0.69 | 0.557955 |
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Target: 5'- cCGCccauGGA-GGCCGCCA-UGCCGCGc -3' miRNA: 3'- -GCGauu-CUUgCCGGCGGUcAUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 62519 | 0.69 | 0.593756 |
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Target: 5'- gCGgaGAGGGCGGUgucggcgcccagccaUGCCAGUACC-CGg -3' miRNA: 3'- -GCgaUUCUUGCCG---------------GCGGUCAUGGcGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 63015 | 0.7 | 0.537178 |
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Target: 5'- gCGCUGugagccauuGAGCGGCUGgcauuCCAgGUACCGCa -3' miRNA: 3'- -GCGAUu--------CUUGCCGGC-----GGU-CAUGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 64228 | 0.67 | 0.689316 |
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Target: 5'- gGC-GAGGAUGGCCGCUGGcaugaaauccccgaACCGCa -3' miRNA: 3'- gCGaUUCUUGCCGGCGGUCa-------------UGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 65118 | 0.76 | 0.24394 |
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Target: 5'- gCGCccugAAGuACGGCCGCCAcaacUACCGCGu -3' miRNA: 3'- -GCGa---UUCuUGCCGGCGGUc---AUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 66926 | 0.69 | 0.568431 |
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Target: 5'- uCGUUGAcucuguGGCGGCC-CUGGUGCCGCGc -3' miRNA: 3'- -GCGAUUc-----UUGCCGGcGGUCAUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 70948 | 0.71 | 0.46594 |
|
Target: 5'- gGCUGAGGcaugcucGCGGCCGCUgagcguggAG-ACCGUGa -3' miRNA: 3'- gCGAUUCU-------UGCCGGCGG--------UCaUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 73276 | 0.74 | 0.319222 |
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Target: 5'- uGCcGGGAggugcGCGGCUggaguGCCAGUACUGCGg -3' miRNA: 3'- gCGaUUCU-----UGCCGG-----CGGUCAUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 74878 | 0.7 | 0.526888 |
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Target: 5'- gGCUAucgAGAccgugACGGCCGCUGGcuacACCGUGa -3' miRNA: 3'- gCGAU---UCU-----UGCCGGCGGUCa---UGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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