Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18404 | 3' | -56.4 | NC_004681.1 | + | 10762 | 0.66 | 0.726761 |
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Target: 5'- aCGC----GACGGCCGCgGGcACCGgGg -3' miRNA: 3'- -GCGauucUUGCCGGCGgUCaUGGCgC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 8751 | 0.66 | 0.767002 |
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Target: 5'- gGCUGGuGACgGGCCGCgAGgaaaucagguCCGCGa -3' miRNA: 3'- gCGAUUcUUG-CCGGCGgUCau--------GGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 7569 | 0.66 | 0.757111 |
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Target: 5'- gGUgGAGGACGGCCG--GGgagGCCGUGa -3' miRNA: 3'- gCGaUUCUUGCCGGCggUCa--UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 7187 | 0.66 | 0.744075 |
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Target: 5'- uCGCgcacGAGAagguacucgagaagGCGGCCgguGCCGGUaacggcgacACCGCGc -3' miRNA: 3'- -GCGa---UUCU--------------UGCCGG---CGGUCA---------UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 5787 | 0.72 | 0.418825 |
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Target: 5'- gGCgauguggAAGAACgcuGGCCGCCccaaccuGGUACUGCGg -3' miRNA: 3'- gCGa------UUCUUG---CCGGCGG-------UCAUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 4158 | 0.68 | 0.600119 |
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Target: 5'- uCGCUGAGAugGcguuCCGCCAGUucaagcugaACCaGUGg -3' miRNA: 3'- -GCGAUUCUugCc---GGCGGUCA---------UGG-CGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 2132 | 0.67 | 0.709192 |
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Target: 5'- cCGCggu--GCGGCCGCCcuccacgaccaccugGCCGCGg -3' miRNA: 3'- -GCGauucuUGCCGGCGGuca------------UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 2034 | 0.72 | 0.410655 |
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Target: 5'- uCGUgGAGGGCGGCCGC----ACCGCGg -3' miRNA: 3'- -GCGaUUCUUGCCGGCGgucaUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 625 | 0.66 | 0.757111 |
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Target: 5'- uCGUUGAGGgacucGCGGCCGCggaAGaugGCCGgGu -3' miRNA: 3'- -GCGAUUCU-----UGCCGGCGg--UCa--UGGCgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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