Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18404 | 3' | -56.4 | NC_004681.1 | + | 16030 | 0.66 | 0.73698 |
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Target: 5'- uGCUGgcaAGGGC-GCCGCCGcuGUcACCGCa -3' miRNA: 3'- gCGAU---UCUUGcCGGCGGU--CA-UGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 18730 | 0.66 | 0.726761 |
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Target: 5'- uCGCcAAGGgcgACGGCgGCgAG-GCCGCGc -3' miRNA: 3'- -GCGaUUCU---UGCCGgCGgUCaUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 30849 | 0.67 | 0.695616 |
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Target: 5'- uCGUgGAGGACGGCaCGCCGc--CCGCc -3' miRNA: 3'- -GCGaUUCUUGCCG-GCGGUcauGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 12243 | 0.67 | 0.706069 |
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Target: 5'- gGCUGAGucgGGUgGcCCGGUGCUGUGg -3' miRNA: 3'- gCGAUUCuugCCGgC-GGUCAUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 64228 | 0.67 | 0.689316 |
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Target: 5'- gGC-GAGGAUGGCCGCUGGcaugaaauccccgaACCGCa -3' miRNA: 3'- gCGaUUCUUGCCGGCGGUCa-------------UGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 61733 | 0.67 | 0.684051 |
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Target: 5'- cCGC-AAGGA-GGCCGCCcucgcgcgccaccAG-ACCGCGg -3' miRNA: 3'- -GCGaUUCUUgCCGGCGG-------------UCaUGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 37648 | 0.67 | 0.663951 |
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Target: 5'- gCGCUccGAcuucuGCGGggccUCGCCAGcgGCCGCGc -3' miRNA: 3'- -GCGAuuCU-----UGCC----GGCGGUCa-UGGCGC- -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 47088 | 0.68 | 0.653328 |
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Target: 5'- cCGCgaacccAAGGACGaGCCGCC---GCCGCu -3' miRNA: 3'- -GCGa-----UUCUUGC-CGGCGGucaUGGCGc -5' |
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| 18404 | 3' | -56.4 | NC_004681.1 | + | 19796 | 1.09 | 0.001318 |
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Target: 5'- cCGCUAAGAACGGCCGCCAGUACCGCGu -3' miRNA: 3'- -GCGAUUCUUGCCGGCGGUCAUGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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