Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18406 | 5' | -56.9 | NC_004681.1 | + | 23941 | 0.66 | 0.764726 |
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Target: 5'- cGGCGCCGGaUCgGCGugcucaaCACGUUCGc -3' miRNA: 3'- -CCGCGGCUcGGgUGCua-----GUGCAAGCc -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 51594 | 0.66 | 0.764726 |
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Target: 5'- cGGUguaGCCGAGCUggcggcaGCGGUCGCccaGUUCGa -3' miRNA: 3'- -CCG---CGGCUCGGg------UGCUAGUG---CAAGCc -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 30270 | 0.66 | 0.77433 |
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Target: 5'- cGGCGUCGAGaCCAcCGAUC-CGU--GGu -3' miRNA: 3'- -CCGCGGCUCgGGU-GCUAGuGCAagCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 12836 | 0.66 | 0.77433 |
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Target: 5'- uGGCGCCGguacguccAGCCUGCag-CugGguacgUCGGg -3' miRNA: 3'- -CCGCGGC--------UCGGGUGcuaGugCa----AGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 6450 | 0.66 | 0.778135 |
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Target: 5'- uGGCGCCcaaGGGCUgcagCGCGGaguUCGCGUacaacucgucggccuUCGGg -3' miRNA: 3'- -CCGCGG---CUCGG----GUGCU---AGUGCA---------------AGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 21351 | 0.66 | 0.783802 |
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Target: 5'- cGGCGUcaCGGGUCCuacCGG-CACGUUCa- -3' miRNA: 3'- -CCGCG--GCUCGGGu--GCUaGUGCAAGcc -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 75641 | 0.66 | 0.783802 |
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Target: 5'- uGGCGCaaaGGGCUCuacCGuGUCGuggauCGUUCGGg -3' miRNA: 3'- -CCGCGg--CUCGGGu--GC-UAGU-----GCAAGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 40240 | 0.66 | 0.783802 |
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Target: 5'- cGGCGUCGcGGCCUGCcuGUCcCGggCGGu -3' miRNA: 3'- -CCGCGGC-UCGGGUGc-UAGuGCaaGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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