Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18406 | 5' | -56.9 | NC_004681.1 | + | 32097 | 0.7 | 0.53817 |
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Target: 5'- cGGCGCCGAucagcgagccgauGCUgGCGAugaUCugGUUCa- -3' miRNA: 3'- -CCGCGGCU-------------CGGgUGCU---AGugCAAGcc -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 33494 | 0.69 | 0.600949 |
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Target: 5'- uGGCGCCGAugcgguucagguGCCaCACGG-CGCGcgCGa -3' miRNA: 3'- -CCGCGGCU------------CGG-GUGCUaGUGCaaGCc -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 4928 | 0.68 | 0.620761 |
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Target: 5'- cGGCGUCGAuGCgCUugGcagccauGUCGCGgUCGGg -3' miRNA: 3'- -CCGCGGCU-CG-GGugC-------UAGUGCaAGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 32139 | 0.68 | 0.621806 |
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Target: 5'- uGCGCUGcuuGCCCAgGAUCACac-CGGc -3' miRNA: 3'- cCGCGGCu--CGGGUgCUAGUGcaaGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 9257 | 0.68 | 0.621806 |
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Target: 5'- aGGCGUCGAagacGUucuCCGCGAUCuccGCGgcggUCGGg -3' miRNA: 3'- -CCGCGGCU----CG---GGUGCUAG---UGCa---AGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 54698 | 0.68 | 0.632251 |
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Target: 5'- gGGUGCCGucgauGUCCACGAUgAUug-CGGg -3' miRNA: 3'- -CCGCGGCu----CGGGUGCUAgUGcaaGCC- -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 37820 | 0.68 | 0.66355 |
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Target: 5'- uGCGCacacaggaGAG-CgACGAUCACGUUCGu -3' miRNA: 3'- cCGCGg-------CUCgGgUGCUAGUGCAAGCc -5' |
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| 18406 | 5' | -56.9 | NC_004681.1 | + | 62100 | 0.73 | 0.388519 |
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Target: 5'- cGCGUCGAGUaggCCGCGAUCugGU-CGa -3' miRNA: 3'- cCGCGGCUCG---GGUGCUAGugCAaGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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