Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18407 | 3' | -60.9 | NC_004681.1 | + | 41944 | 0.67 | 0.423303 |
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Target: 5'- cAGGUCGGcuccgcgGAGAuCGGCCCGCUgcaGGGAa -3' miRNA: 3'- -UUCGGCC-------UUCU-GCCGGGCGGa--CUCUg -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 42284 | 0.67 | 0.415182 |
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Target: 5'- cGGCCuGGAAGAaGGCCCaGCUcGGGAg -3' miRNA: 3'- uUCGG-CCUUCUgCCGGG-CGGaCUCUg -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 44239 | 0.67 | 0.424211 |
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Target: 5'- cGGCCGGugAGGGCcaGGCCCGCggccucgggggUGAGGCa -3' miRNA: 3'- uUCGGCC--UUCUG--CCGGGCGg----------ACUCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 48778 | 0.66 | 0.442617 |
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Target: 5'- cGGCCcauGGGAGcUGGCCCGCCaGGGu- -3' miRNA: 3'- uUCGG---CCUUCuGCCGGGCGGaCUCug -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 49011 | 0.67 | 0.406271 |
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Target: 5'- -uGCUGGAuggucuuguacgGGACGGuCCCGCguagacgUGAGACa -3' miRNA: 3'- uuCGGCCU------------UCUGCC-GGGCGg------ACUCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 58228 | 0.66 | 0.442617 |
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Target: 5'- gAGGCCGGggGACua-CCGCCaugGAGuCu -3' miRNA: 3'- -UUCGGCCuuCUGccgGGCGGa--CUCuG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 59705 | 0.68 | 0.371024 |
|
Target: 5'- -uGCUGGGAGuGCGGCCUGCCaacacucggaaccUGGGuCg -3' miRNA: 3'- uuCGGCCUUC-UGCCGGGCGG-------------ACUCuG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 59755 | 0.68 | 0.354604 |
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Target: 5'- uGGCUGGAagcccaccgcuucGGAC-GCCCGCUUGuGGACg -3' miRNA: 3'- uUCGGCCU-------------UCUGcCGGGCGGAC-UCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 61834 | 0.66 | 0.461465 |
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Target: 5'- uGGCgCGcGAGGGCGGCCUcCUUGcGGACg -3' miRNA: 3'- uUCG-GC-CUUCUGCCGGGcGGAC-UCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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