Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18407 | 3' | -60.9 | NC_004681.1 | + | 2923 | 0.68 | 0.371859 |
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Target: 5'- uGGCUGGgcGACGGCaCCGCCa----- -3' miRNA: 3'- uUCGGCCuuCUGCCG-GGCGGacucug -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 59705 | 0.68 | 0.371024 |
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Target: 5'- -uGCUGGGAGuGCGGCCUGCCaacacucggaaccUGGGuCg -3' miRNA: 3'- uuCGGCCUUC-UGCCGGGCGG-------------ACUCuG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 23424 | 0.68 | 0.371024 |
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Target: 5'- -uGCCGGAAGAggUGGCCCagaagcgaauucgGCCcgaUGAGAa -3' miRNA: 3'- uuCGGCCUUCU--GCCGGG-------------CGG---ACUCUg -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 367 | 0.68 | 0.355413 |
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Target: 5'- --uCCGcGAGGACGaGCCCGCggucaagacCUGGGGCa -3' miRNA: 3'- uucGGC-CUUCUGC-CGGGCG---------GACUCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 59755 | 0.68 | 0.354604 |
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Target: 5'- uGGCUGGAagcccaccgcuucGGAC-GCCCGCUUGuGGACg -3' miRNA: 3'- uUCGGCCU-------------UCUGcCGGGCGGAC-UCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 18148 | 0.69 | 0.331719 |
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Target: 5'- --aUCGGAAGaccGCGGCgCUGCCgUGAGGCg -3' miRNA: 3'- uucGGCCUUC---UGCCG-GGCGG-ACUCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 1278 | 0.7 | 0.287878 |
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Target: 5'- -uGUCGGAGGGC-GCCCaggaaGUCUGGGACg -3' miRNA: 3'- uuCGGCCUUCUGcCGGG-----CGGACUCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 34170 | 0.74 | 0.145193 |
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Target: 5'- aAAGCC-GAAGGC--CCCGCCUGAGACu -3' miRNA: 3'- -UUCGGcCUUCUGccGGGCGGACUCUG- -5' |
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| 18407 | 3' | -60.9 | NC_004681.1 | + | 20384 | 1.06 | 0.000629 |
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Target: 5'- gAAGCCGGAAGACGGCCCGCCUGAGACc -3' miRNA: 3'- -UUCGGCCUUCUGCCGGGCGGACUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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