Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18409 | 5' | -46.1 | NC_004681.1 | + | 43771 | 0.66 | 0.998716 |
Target: 5'- uGGAggggUUGAUcGGCgGGCUGAA-GGGCg -3' miRNA: 3'- -CCUaa--AGCUAaUUG-CCGGCUUaCCUG- -5' |
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18409 | 5' | -46.1 | NC_004681.1 | + | 69273 | 0.66 | 0.998716 |
Target: 5'- uGAUgggcCGAUcUGAUGGaCCGcGUGGACg -3' miRNA: 3'- cCUAaa--GCUA-AUUGCC-GGCuUACCUG- -5' |
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18409 | 5' | -46.1 | NC_004681.1 | + | 54744 | 0.66 | 0.999161 |
Target: 5'- cGGGUguagUCGuagggugAGCGGCCGuucacggGGAUa -3' miRNA: 3'- -CCUAa---AGCuaa----UUGCCGGCuua----CCUG- -5' |
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18409 | 5' | -46.1 | NC_004681.1 | + | 7355 | 0.66 | 0.999161 |
Target: 5'- -----cCGAggugGACGGCCGcgucGUGGAUg -3' miRNA: 3'- ccuaaaGCUaa--UUGCCGGCu---UACCUG- -5' |
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18409 | 5' | -46.1 | NC_004681.1 | + | 47333 | 0.66 | 0.999329 |
Target: 5'- cGGAUggucUC-AUUGucgGCGGUgCGGAUGGACu -3' miRNA: 3'- -CCUAa---AGcUAAU---UGCCG-GCUUACCUG- -5' |
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18409 | 5' | -46.1 | NC_004681.1 | + | 8630 | 0.66 | 0.999329 |
Target: 5'- gGGGUUcUGGccGAUGGCCGcggGGGCg -3' miRNA: 3'- -CCUAAaGCUaaUUGCCGGCuuaCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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