Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18409 | 5' | -46.1 | NC_004681.1 | + | 62534 | 0.72 | 0.953421 |
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Target: 5'- cGGGUUUCcug-GGCGGCgGAGaGGGCg -3' miRNA: 3'- -CCUAAAGcuaaUUGCCGgCUUaCCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 8962 | 0.72 | 0.94885 |
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Target: 5'- uGGAUgccUUCGAgcaGGCGGCCc--UGGACg -3' miRNA: 3'- -CCUA---AAGCUaa-UUGCCGGcuuACCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 19617 | 0.72 | 0.938837 |
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Target: 5'- cGGcgUggUCGA-UGACGGCgaGggUGGGCa -3' miRNA: 3'- -CCuaA--AGCUaAUUGCCGg-CuuACCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 12055 | 0.79 | 0.67919 |
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Target: 5'- ----aUCGGcgUUGACGGCCGcAUGGACg -3' miRNA: 3'- ccuaaAGCU--AAUUGCCGGCuUACCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 2566 | 0.79 | 0.645098 |
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Target: 5'- cGGAguaUUCGAc--GCGGCCGAagaGUGGGCg -3' miRNA: 3'- -CCUa--AAGCUaauUGCCGGCU---UACCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 21178 | 1.15 | 0.006589 |
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Target: 5'- uGGAUUUCGAUUAACGGCCGAAUGGACa -3' miRNA: 3'- -CCUAAAGCUAAUUGCCGGCUUACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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