Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18409 | 5' | -46.1 | NC_004681.1 | + | 62534 | 0.72 | 0.953421 |
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Target: 5'- cGGGUUUCcug-GGCGGCgGAGaGGGCg -3' miRNA: 3'- -CCUAAAGcuaaUUGCCGgCUUaCCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 63550 | 0.67 | 0.997208 |
|
Target: 5'- uGGGUUcUUGAUc--CGGCCGAGgcUGGAa -3' miRNA: 3'- -CCUAA-AGCUAauuGCCGGCUU--ACCUg -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 65231 | 0.67 | 0.99768 |
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Target: 5'- cGGAgacgCGGUaguugUGGCGGCCGuacuucaGGGCg -3' miRNA: 3'- -CCUaaa-GCUA-----AUUGCCGGCuua----CCUG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 66399 | 0.71 | 0.97215 |
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Target: 5'- aGGAaggUCGGUauGCaGCCGGAUGGGu -3' miRNA: 3'- -CCUaa-AGCUAauUGcCGGCUUACCUg -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 69015 | 0.67 | 0.998427 |
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Target: 5'- ---cUUCGGUggcGGCGGCCGugacGAUGGuCa -3' miRNA: 3'- ccuaAAGCUAa--UUGCCGGC----UUACCuG- -5' |
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| 18409 | 5' | -46.1 | NC_004681.1 | + | 69273 | 0.66 | 0.998716 |
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Target: 5'- uGAUgggcCGAUcUGAUGGaCCGcGUGGACg -3' miRNA: 3'- cCUAaa--GCUA-AUUGCC-GGCuUACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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