Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18410 | 3' | -57.3 | NC_004681.1 | + | 32762 | 0.67 | 0.636968 |
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Target: 5'- cGGCC-CGGUCgGCaAUCcgCugGGCCGc -3' miRNA: 3'- -CUGGaGCCAGaCG-UAGa-GugCCGGUc -5' |
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| 18410 | 3' | -57.3 | NC_004681.1 | + | 23826 | 0.67 | 0.626294 |
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Target: 5'- -uCCUCGGgCaGCGUCUCGgaccCGGUCAGc -3' miRNA: 3'- cuGGAGCCaGaCGUAGAGU----GCCGGUC- -5' |
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| 18410 | 3' | -57.3 | NC_004681.1 | + | 12205 | 0.67 | 0.615625 |
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Target: 5'- cGACCUUGGUCaGCGagacgCGCGGgCGGa -3' miRNA: 3'- -CUGGAGCCAGaCGUaga--GUGCCgGUC- -5' |
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| 18410 | 3' | -57.3 | NC_004681.1 | + | 17830 | 0.7 | 0.461365 |
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Target: 5'- cGACCUCGGUguugugCUGCuugacCGCGGCCGu -3' miRNA: 3'- -CUGGAGCCA------GACGuaga-GUGCCGGUc -5' |
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| 18410 | 3' | -57.3 | NC_004681.1 | + | 68984 | 0.7 | 0.461365 |
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Target: 5'- cGCCUCGaug-GCGUCggcCGCGGCCAGu -3' miRNA: 3'- cUGGAGCcagaCGUAGa--GUGCCGGUC- -5' |
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| 18410 | 3' | -57.3 | NC_004681.1 | + | 40252 | 0.71 | 0.396485 |
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Target: 5'- cGCCUCGaUCUGCGgcgUCGCGGCCu- -3' miRNA: 3'- cUGGAGCcAGACGUag-AGUGCCGGuc -5' |
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| 18410 | 3' | -57.3 | NC_004681.1 | + | 21237 | 1.07 | 0.001207 |
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Target: 5'- gGACCUCGGUCUGCAUCUCACGGCCAGc -3' miRNA: 3'- -CUGGAGCCAGACGUAGAGUGCCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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