Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18412 | 3' | -56.5 | NC_004681.1 | + | 23234 | 0.66 | 0.743006 |
Target: 5'- cAACGACCUGGcUCaggaUGGcuuUGGGGAGGg -3' miRNA: 3'- -UUGCUGGACU-AG----ACCcu-GCCCCUUCa -5' |
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18412 | 3' | -56.5 | NC_004681.1 | + | 33928 | 0.67 | 0.658797 |
Target: 5'- --aGAgCUGGUa-GGGACGGGGAAu- -3' miRNA: 3'- uugCUgGACUAgaCCCUGCCCCUUca -5' |
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18412 | 3' | -56.5 | NC_004681.1 | + | 14983 | 0.67 | 0.637304 |
Target: 5'- cGGCGACCUGcgaggCgGGGAUGuGGAAGUc -3' miRNA: 3'- -UUGCUGGACua---GaCCCUGCcCCUUCA- -5' |
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18412 | 3' | -56.5 | NC_004681.1 | + | 51986 | 0.69 | 0.530944 |
Target: 5'- uGCGGCCaUGAUCUGcGaGAgCGGGGAccaGGUg -3' miRNA: 3'- uUGCUGG-ACUAGAC-C-CU-GCCCCU---UCA- -5' |
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18412 | 3' | -56.5 | NC_004681.1 | + | 22192 | 1.04 | 0.002245 |
Target: 5'- cAACGACCUGAUCUGGGACGGGGAAGUu -3' miRNA: 3'- -UUGCUGGACUAGACCCUGCCCCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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