Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18412 | 5' | -54 | NC_004681.1 | + | 33943 | 0.66 | 0.870987 |
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Target: 5'- --cCUUCACgUGUUCCuagagcugguaGGGACGGGg- -3' miRNA: 3'- cuuGAAGUGgGCAAGG-----------CUCUGCCCac -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 50938 | 0.67 | 0.838114 |
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Target: 5'- -cGCUUCACCUcUUCggCGAGcUGGGUGg -3' miRNA: 3'- cuUGAAGUGGGcAAG--GCUCuGCCCAC- -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 22334 | 0.67 | 0.801911 |
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Target: 5'- uGACaaCACCCGUcUCGGaACGGGUGa -3' miRNA: 3'- cUUGaaGUGGGCAaGGCUcUGCCCAC- -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 26001 | 0.68 | 0.762975 |
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Target: 5'- cAACUUCGCCCuGUUCCcGaAGuCGGGa- -3' miRNA: 3'- cUUGAAGUGGG-CAAGG-C-UCuGCCCac -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 12230 | 0.68 | 0.75289 |
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Target: 5'- ----gUgGCCCGUUCUGGGcugaguCGGGUGg -3' miRNA: 3'- cuugaAgUGGGCAAGGCUCu-----GCCCAC- -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 18908 | 0.77 | 0.296413 |
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Target: 5'- uGACUUCACCC---UCGGGAUGGGUGg -3' miRNA: 3'- cUUGAAGUGGGcaaGGCUCUGCCCAC- -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 49301 | 0.77 | 0.275239 |
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Target: 5'- -cACUUCAgCCGUUCCGAGACcaucuuGGUGa -3' miRNA: 3'- cuUGAAGUgGGCAAGGCUCUGc-----CCAC- -5' |
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| 18412 | 5' | -54 | NC_004681.1 | + | 22227 | 1.08 | 0.002454 |
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Target: 5'- cGAACUUCACCCGUUCCGAGACGGGUGu -3' miRNA: 3'- -CUUGAAGUGGGCAAGGCUCUGCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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