Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18413 | 3' | -52 | NC_004681.1 | + | 40105 | 0.66 | 0.932613 |
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Target: 5'- uGACugcaccccgGGgGUCGccAGGUCGGUGAccgCCc -3' miRNA: 3'- gCUG---------UCgCAGC--UCCAGCCACUuuaGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 4913 | 0.66 | 0.942786 |
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Target: 5'- uGGCAGCcauGUCGcGGUCGGguuuGUCa -3' miRNA: 3'- gCUGUCG---CAGCuCCAGCCacuuUAGg -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 47126 | 0.66 | 0.942786 |
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Target: 5'- uCGuCGGgGUCGGGcucGUCGGUGggGa-- -3' miRNA: 3'- -GCuGUCgCAGCUC---CAGCCACuuUagg -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 23907 | 0.66 | 0.942786 |
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Target: 5'- aCGACuuucGCGucaUCGAGGUagaaggugccugCGGUGGcuUCCu -3' miRNA: 3'- -GCUGu---CGC---AGCUCCA------------GCCACUuuAGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 9301 | 0.66 | 0.942786 |
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Target: 5'- -cACAGCGuUCGGGGUCuGGaacagggucagcUGGAuGUCCa -3' miRNA: 3'- gcUGUCGC-AGCUCCAG-CC------------ACUU-UAGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 45206 | 0.66 | 0.946096 |
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Target: 5'- gCGACGGCGUCGGccccGGUcuugagcgagaccgCGGcGAGggCCu -3' miRNA: 3'- -GCUGUCGCAGCU----CCA--------------GCCaCUUuaGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 23821 | 0.66 | 0.947476 |
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Target: 5'- gGGCAGCGUCucGGaccCGGUcagcucGAAUCCg -3' miRNA: 3'- gCUGUCGCAGcuCCa--GCCAc-----UUUAGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 52037 | 0.66 | 0.951904 |
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Target: 5'- uGugGGUGUCGAGGuauuccUCGacgcgcucccacGUGGAGUUCu -3' miRNA: 3'- gCugUCGCAGCUCC------AGC------------CACUUUAGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 26906 | 0.66 | 0.951904 |
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Target: 5'- aCGuCAGCGUUGGccgccgCGGUGAAGUUg -3' miRNA: 3'- -GCuGUCGCAGCUcca---GCCACUUUAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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