Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18413 | 3' | -52 | NC_004681.1 | + | 9301 | 0.66 | 0.942786 |
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Target: 5'- -cACAGCGuUCGGGGUCuGGaacagggucagcUGGAuGUCCa -3' miRNA: 3'- gcUGUCGC-AGCUCCAG-CC------------ACUU-UAGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 9069 | 0.77 | 0.422925 |
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Target: 5'- cCGACAGCGagGAGGccgaggaggCGGUGAAG-CCg -3' miRNA: 3'- -GCUGUCGCagCUCCa--------GCCACUUUaGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 7443 | 0.67 | 0.915347 |
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Target: 5'- aGGCAGCGUCGAGGaUC--UGAccGA-CCg -3' miRNA: 3'- gCUGUCGCAGCUCC-AGccACU--UUaGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 7193 | 0.73 | 0.650412 |
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Target: 5'- aCGAugcCAGCGUUGAGGaguuccCGGUGggGggugCCg -3' miRNA: 3'- -GCU---GUCGCAGCUCCa-----GCCACuuUa---GG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 6896 | 0.71 | 0.745898 |
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Target: 5'- uCGACGGgGUUGAGaGcaccgCGGUGAAG-CCg -3' miRNA: 3'- -GCUGUCgCAGCUC-Ca----GCCACUUUaGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 6666 | 0.68 | 0.873716 |
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Target: 5'- uGACGGCG-CGcAGGugcuUCGGUGAcgacUCCu -3' miRNA: 3'- gCUGUCGCaGC-UCC----AGCCACUuu--AGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 4913 | 0.66 | 0.942786 |
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Target: 5'- uGGCAGCcauGUCGcGGUCGGguuuGUCa -3' miRNA: 3'- gCUGUCG---CAGCuCCAGCCacuuUAGg -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 4177 | 0.68 | 0.888616 |
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Target: 5'- gCGACccAGCG-CGGGGUUGGcgUGggG-CCa -3' miRNA: 3'- -GCUG--UCGCaGCUCCAGCC--ACuuUaGG- -5' |
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| 18413 | 3' | -52 | NC_004681.1 | + | 408 | 0.68 | 0.873716 |
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Target: 5'- cCGGCAGCccGUgcuucCGcGGGUCGGUGAugacaguUCCa -3' miRNA: 3'- -GCUGUCG--CA-----GC-UCCAGCCACUuu-----AGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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