Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18417 | 3' | -60.2 | NC_004681.1 | + | 51590 | 0.7 | 0.305964 |
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Target: 5'- gUAGCCgAGCUGGcggCAGCGGUCg--- -3' miRNA: 3'- -GUCGGgUCGACCua-GUCGCCGGacug -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 9646 | 0.7 | 0.291614 |
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Target: 5'- --cUUCAGCUGGAUCAGCuggaaGGCCgaggGGCg -3' miRNA: 3'- gucGGGUCGACCUAGUCG-----CCGGa---CUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 30964 | 0.71 | 0.264504 |
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Target: 5'- gGGCuCCAGC-GGGUCGGCGGgC-GGCg -3' miRNA: 3'- gUCG-GGUCGaCCUAGUCGCCgGaCUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 36887 | 0.72 | 0.20567 |
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Target: 5'- -uGCCUAGUcagcgGGGggcgggcCAGCGGCCUGGCa -3' miRNA: 3'- guCGGGUCGa----CCUa------GUCGCCGGACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 9872 | 0.73 | 0.188894 |
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Target: 5'- uCAGCCCGGUuggcguaggcggagUGGAccgcggggaugaUCAGCGGCUUGGg -3' miRNA: 3'- -GUCGGGUCG--------------ACCU------------AGUCGCCGGACUg -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 8566 | 0.74 | 0.158508 |
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Target: 5'- gCAGCCguGCcgGGGUCAGCcGCCUuGGCg -3' miRNA: 3'- -GUCGGguCGa-CCUAGUCGcCGGA-CUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 9464 | 0.76 | 0.114941 |
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Target: 5'- -uGCCCAGCgGGAUCAGCGGgguggUGACa -3' miRNA: 3'- guCGGGUCGaCCUAGUCGCCgg---ACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 23848 | 1.09 | 0.000405 |
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Target: 5'- uCAGCCCAGCUGGAUCAGCGGCCUGACg -3' miRNA: 3'- -GUCGGGUCGACCUAGUCGCCGGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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