Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18417 | 3' | -60.2 | NC_004681.1 | + | 51614 | 0.69 | 0.32085 |
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Target: 5'- -cGCCCAGCUcguaGAgaagUGGCGGaCCUGGCa -3' miRNA: 3'- guCGGGUCGAc---CUa---GUCGCC-GGACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 54504 | 0.67 | 0.438791 |
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Target: 5'- aGGCCaagAGCUggucccaGGAUCGGUGGCCcGAg -3' miRNA: 3'- gUCGGg--UCGA-------CCUAGUCGCCGGaCUg -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 58753 | 0.67 | 0.421239 |
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Target: 5'- uGGCCC-GC-GGcgCAgGCGGCCgUGGCg -3' miRNA: 3'- gUCGGGuCGaCCuaGU-CGCCGG-ACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 59698 | 0.69 | 0.32085 |
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Target: 5'- -cGCCCccuGCUGGGaguGCGGCCUGcCa -3' miRNA: 3'- guCGGGu--CGACCUaguCGCCGGACuG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 60015 | 0.66 | 0.467331 |
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Target: 5'- aAGCCCGG-UGGGcaccugcucgccuUCGGCGGCUcccgcaccUGGCa -3' miRNA: 3'- gUCGGGUCgACCU-------------AGUCGCCGG--------ACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 60488 | 0.67 | 0.449141 |
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Target: 5'- -uGCgCAGCgGGAUUAGUGGCggcaaUGGCg -3' miRNA: 3'- guCGgGUCGaCCUAGUCGCCGg----ACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 64766 | 0.67 | 0.430424 |
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Target: 5'- aGGCCCAGCUuggcgGGAUCGucCGGUaUGGCg -3' miRNA: 3'- gUCGGGUCGA-----CCUAGUc-GCCGgACUG- -5' |
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| 18417 | 3' | -60.2 | NC_004681.1 | + | 65077 | 0.66 | 0.517914 |
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Target: 5'- -cGCUCGGgUGGucgccgaGGuCGGCCUGGCa -3' miRNA: 3'- guCGGGUCgACCuag----UC-GCCGGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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