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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18420 | 5' | -56.6 | NC_004681.1 | + | 41939 | 0.72 | 0.389575 |
Target: 5'- cGGCuCCGCGGAGAUcggCCCGcugcagggaaaUGCCCc -3' miRNA: 3'- aCCGcGGUGCCUUUAa--GGGU-----------ACGGGc -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 6613 | 0.72 | 0.380872 |
Target: 5'- aGGCGCCGCGGcucggucUUCCCcgaGUCCGu -3' miRNA: 3'- aCCGCGGUGCCuuu----AAGGGua-CGGGC- -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 46780 | 0.72 | 0.3723 |
Target: 5'- cGGCGUCACGGuca-UCCCGcGCgCCGu -3' miRNA: 3'- aCCGCGGUGCCuuuaAGGGUaCG-GGC- -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 38322 | 0.72 | 0.363861 |
Target: 5'- aUGGCGCCgccGCGGGAcgUCUUcgGCCg- -3' miRNA: 3'- -ACCGCGG---UGCCUUuaAGGGuaCGGgc -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 55131 | 0.73 | 0.316051 |
Target: 5'- uUGGCGCUACGucaGAAGUUCCCcggGCgCGa -3' miRNA: 3'- -ACCGCGGUGC---CUUUAAGGGua-CGgGC- -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 18195 | 0.77 | 0.191253 |
Target: 5'- gGGCGCCGaugcCGGGGAUgCCgAUGCCCa -3' miRNA: 3'- aCCGCGGU----GCCUUUAaGGgUACGGGc -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 34824 | 0.81 | 0.102716 |
Target: 5'- cGGCgGCCgaagcgcGCGGGAAUUCCCAaGCCCGc -3' miRNA: 3'- aCCG-CGG-------UGCCUUUAAGGGUaCGGGC- -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 51223 | 0.81 | 0.09747 |
Target: 5'- cGGcCGUCAUGGAGAUUCUCAaGCCCGa -3' miRNA: 3'- aCC-GCGGUGCCUUUAAGGGUaCGGGC- -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 18451 | 0.82 | 0.087242 |
Target: 5'- aUGGCGCCAcCGGggGUgaaCCCGaGCCCGc -3' miRNA: 3'- -ACCGCGGU-GCCuuUAa--GGGUaCGGGC- -5' |
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18420 | 5' | -56.6 | NC_004681.1 | + | 24788 | 1.08 | 0.001303 |
Target: 5'- cUGGCGCCACGGAAAUUCCCAUGCCCGc -3' miRNA: 3'- -ACCGCGGUGCCUUUAAGGGUACGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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