Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18424 | 3' | -56.8 | NC_004681.1 | + | 56412 | 0.68 | 0.625214 |
Target: 5'- gCCUUgGCGCCGUgGGuGUGCuugaccGGCaGCu -3' miRNA: 3'- -GGAAgCGCGGCGgUCuCAUG------CCGaUG- -5' |
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18424 | 3' | -56.8 | NC_004681.1 | + | 42918 | 0.68 | 0.615645 |
Target: 5'- aCCgUgGCGgCGCCAGAGaagguauucggcuucGCGGUUGCa -3' miRNA: 3'- -GGaAgCGCgGCGGUCUCa--------------UGCCGAUG- -5' |
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18424 | 3' | -56.8 | NC_004681.1 | + | 17637 | 0.68 | 0.602906 |
Target: 5'- ----gGUGCCGCCAGGGaGCguggccugcauguGGCUGCu -3' miRNA: 3'- ggaagCGCGGCGGUCUCaUG-------------CCGAUG- -5' |
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18424 | 3' | -56.8 | NC_004681.1 | + | 3133 | 0.7 | 0.51033 |
Target: 5'- cCCUUgGCGCCGC--GGGUGCGGgcaUUGCc -3' miRNA: 3'- -GGAAgCGCGGCGguCUCAUGCC---GAUG- -5' |
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18424 | 3' | -56.8 | NC_004681.1 | + | 75520 | 0.7 | 0.470614 |
Target: 5'- ---cUGCGCCGC--GAGUACGGCcGCg -3' miRNA: 3'- ggaaGCGCGGCGguCUCAUGCCGaUG- -5' |
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18424 | 3' | -56.8 | NC_004681.1 | + | 20661 | 0.72 | 0.362094 |
Target: 5'- uCCgccaGCGCCGCCAuuuGUGCGGCg-- -3' miRNA: 3'- -GGaag-CGCGGCGGUcu-CAUGCCGaug -5' |
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18424 | 3' | -56.8 | NC_004681.1 | + | 28921 | 0.66 | 0.749541 |
Target: 5'- gCCU--GCGCCuGCCAGucGGUAUaugacgaGGCUGCc -3' miRNA: 3'- -GGAagCGCGG-CGGUC--UCAUG-------CCGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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