Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18429 | 3' | -45 | NC_004681.1 | + | 56587 | 0.71 | 0.976835 |
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Target: 5'- ---cCGCAAGGuGAUcaagGACGUCAacaACCCc -3' miRNA: 3'- cauuGCGUUCC-CUAa---UUGUAGU---UGGG- -5' |
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| 18429 | 3' | -45 | NC_004681.1 | + | 50571 | 0.72 | 0.959867 |
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Target: 5'- uUGGCGCAGgacacggucGGGGUgGGCGUC-GCCCa -3' miRNA: 3'- cAUUGCGUU---------CCCUAaUUGUAGuUGGG- -5' |
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| 18429 | 3' | -45 | NC_004681.1 | + | 26328 | 0.73 | 0.951145 |
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Target: 5'- --cGCGUAGGGGGcgGGCAUCGccGCCg -3' miRNA: 3'- cauUGCGUUCCCUaaUUGUAGU--UGGg -5' |
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| 18429 | 3' | -45 | NC_004681.1 | + | 16623 | 0.73 | 0.951145 |
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Target: 5'- ---cCGCGAuGGAggcggcgGGCAUCAGCCCg -3' miRNA: 3'- cauuGCGUUcCCUaa-----UUGUAGUUGGG- -5' |
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| 18429 | 3' | -45 | NC_004681.1 | + | 65200 | 0.75 | 0.896208 |
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Target: 5'- -gGACGCcugguggGAGGGucagGAUAUCGACCCc -3' miRNA: 3'- caUUGCG-------UUCCCuaa-UUGUAGUUGGG- -5' |
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| 18429 | 3' | -45 | NC_004681.1 | + | 18868 | 0.79 | 0.712847 |
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Target: 5'- -cGACGCAu-GGAUUuccuACGUCAGCCCa -3' miRNA: 3'- caUUGCGUucCCUAAu---UGUAGUUGGG- -5' |
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| 18429 | 3' | -45 | NC_004681.1 | + | 28459 | 1.15 | 0.007488 |
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Target: 5'- uGUAACGCAAGGGAUUAACAUCAACCCg -3' miRNA: 3'- -CAUUGCGUUCCCUAAUUGUAGUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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